Angiotensin II ELISA Kit

Produktnummer ABIN8089438

Rind (Kuh) Angiotensin II ELISA Kit, Colorimetric Assay für die Quantifizierung von Rind (Kuh) Angiotensin II.

Kurzübersicht für Angiotensin II ELISA Kit (ABIN8089438)

Target: Angiotensin II (Ang II)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 1.563 pg/mL - 100 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails Angiotensin II ELISA Kit

Untere Nachweisgrenze: 1.563 pg/mL

Verwendungszweck: Bovine Angiotensin II (Angiotensin II) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with ANGII , no obvious cross reaction with other analogues.

Sensitivität: 0.938 pg/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP-Antigen(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antibody

Testdauer: 1 h

Plattentyp: Pre-coated

Protokoll: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. (Equilibrate the whole bottle of TMB for 30 min at 37 °C.) Washing: Wash the plate five times and immerse for 1 min each time. Step 3: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu Angiotensin II

Target: Angiotensin II (Ang II)

Andere Bezeichnung: ANG II

Hintergrund: ANG II, Angiotensin II

UniProt: P01017

Pathways: JAK-STAT Signalweg, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity