VEGFA ELISA Kit

Produktnummer ABIN8088938

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte und Maus VEGFA.

Kurzübersicht für VEGFA ELISA Kit (ABIN8088938)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails VEGFA ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Mouse/Rat VEGFA ELISA Kit for detecting Vascular Endothelial Growth Factor A in Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates

Analytische Methode: Quantitative

Sensitivität: 5.8 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: Vascular Endothelial Growth Factor A

Hintergrund: Gene Aliases: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling