TGFB1 CLIA Kit

Produktnummer ABIN8088182

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Ratte TGFB1.

Kurzübersicht für TGFB1 CLIA Kit (ABIN8088182)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 2.74 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate

Produktdetails TGFB1 CLIA Kit

Untere Nachweisgrenze: 2.74 pg/mL

Verwendungszweck: Rat Transforming Growth Factor Beta 1 (TGFb1) CLIA Kit for detecting TGFb1 in serum, platelet-poor plasma, tissue homogenates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.93 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: Transforming Growth Factor Beta 1

Hintergrund: Gene Aliases: TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

Gen-ID: 59086

UniProt: P17246

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints