TCF7L2 ELISA Kit

Produktnummer ABIN8088118

Human TCF7L2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human TCF7L2.

Kurzübersicht für TCF7L2 ELISA Kit (ABIN8088118)

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails TCF7L2 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human Transcription Factor 7 Like Protein 2 (TCF7L2) ELISA Kit for detecting TCF7L2 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.054 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu TCF7L2

Target: TCF7L2 (Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2))

Andere Bezeichnung: Transcription Factor 7 Like Protein 2

Hintergrund: Gene Aliases: TCF4, HMG box transcription factor 4

Gen-ID: 6934

UniProt: Q9NQB0

Pathways: WNT Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus