SIRT1 ELISA Kit
Kurzübersicht für SIRT1 ELISA Kit (ABIN8087000)
Target
Alle SIRT1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.16 ng/mL
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Verwendungszweck
- Rat SIRT1 (Sirtuin 1) ELISA Kit for detecting SIRT1 in serum, plasma
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Analytische Methode
- Quantitative
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Sensitivität
- 0.1 ng/mL
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Bestandteile
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Benötigtes Material
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Sirtuin 1 (SIRT1) ELISA Kit
SIRT1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Maus Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
SensoLyte® Green Sirt1 Assay KitFRET Fluorometric
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 2-8°C for 12 months, Long term: see manual.
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Haltbarkeit
- 12 months
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- SIRT1 (Sirtuin 1 (SIRT1))
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Andere Bezeichnung
- SIRT1
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Hintergrund
- Gene Aliases: SIR2L1, SIR2alpha, sir2-like 1, sirtuin 1, Silent Mating Type Information Regulation 2 Homolog 1
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UniProt
- A0A0G2
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Pathways
- MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
Target Alle SIRT1 ELISA Kits anzeigen
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