SensoLyte® 520 FRET Sirt1 Assay Kit

Produktnummer ABIN1882553

Produktdetails

Target: SIRT1 (Sirtuin 1 (SIRT1))

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 FRET SIRT1 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 1 activity using FRET substrate. In the first step, an acetylated substrate is incubated with SIRT1 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490/520 nm. This kit can be used for high throughput screening of SIRT1 inhibitors/activators. The long wavelength fluorescence of FRET substrate is less interfered by the autofluorescence of cell components and test compounds. The nuclear extract HDAC (containing HDACs 1 and 2) does not deacetylate FRET sirtuin substrate. Assays are performed in a standard 96-well format.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Antigendetails

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: Sirt1 (SIRT1 Produkte)

Synonyme: sirtuin 1 Kit, AA673258 Kit, SIR2L1 Kit, Sir2 Kit, Sir2a Kit, Sir2alpha Kit, sir2l1 Kit, sirtuin Kit, SIR2 Kit, sirtuin 1 Kit, sirtuin 1 L homeolog Kit, SIRT1 Kit, SRT1 Kit, Sirt1 Kit, sirt1.L Kit

Hintergrund: Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD as a cofactor. Sirtuin 1 (SIRT1) is involved in regulation of tumor suppressor p53 and inhibition of apoptosis. It also represents a target for treatment of age-related diseases and type II diabetes.