SIRT1 CLIA Kit

Produktnummer ABIN8086998

Das Ratte SIRT1 ELISA Kit (ABIN8086998) ist ein Chemiluminescent ELISA Kit zur Detektion von Ratte SIRT1.

Kurzübersicht für SIRT1 CLIA Kit (ABIN8086998)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 27.4 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails SIRT1 CLIA Kit

Untere Nachweisgrenze: 27.4 pg/mL

Verwendungszweck: Rat Sirtuin 1 (SIRT1) CLIA Kit for detecting SIRT1 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 10.5 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: Sirtuin 1

Hintergrund: Gene Aliases: SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment

UniProt: A0A8I6

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling