STAT5B ELISA Kit

Produktnummer ABIN8086974

Das Human, Ratte und Maus STAT5B ELISA Kit (ABIN8086974) ist ein Colorimetric ELISA Kit zur Detektion von Human, Ratte und Maus STAT5B.

Kurzübersicht für STAT5B ELISA Kit (ABIN8086974)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails STAT5B ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: Human/Mouse/Rat Signal Transducer And Activator Of Transcription 5B (STAT5B) ELISA Kit for detecting STAT5B in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.125 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Andere Bezeichnung: Signal Transducer And Activator Of Transcription 5B

Hintergrund: Gene Aliases: STAT5-B

Gen-ID: 6777

UniProt: P51692

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation