PTK2B ELISA Kit

Produktnummer ABIN8086033

Human, Maus und Ratte PTK2B ELISA Kit, Colorimetric Assay für die Quantifizierung von Human, Maus und Ratte PTK2B.

Kurzübersicht für PTK2B ELISA Kit (ABIN8086033)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Bindungsspezifität: pTyr579

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails PTK2B ELISA Kit

Verwendungszweck: Phospho-PYK2 (Tyr579) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PYK2 (Tyr579) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Andere Bezeichnung: PYK2

Hintergrund: Gene Aliases: CADTK, CAK beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FAK2, Focal adhesion kinase 2, kinase Pyk2, Proline-rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK2B, RAFTK, Related adhesion focal tyrosine kinase

Gen-ID: 2185

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling