PTK2B ELISA Kit

Produktnummer ABIN2748576

Produktdetails PTK2B ELISA Kit

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Bindungsspezifität: pTyr402

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-PYK2 (Y402) ELISA Kit. This assay semi-quantitatively measures phophorylated PYK2 (Tyr402) in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes PYK2 phosphorylated at Tyrosine-402.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents and samples as instructed in the manual.Add 100 μL of sample or positive control to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared primary antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared 1X HRP-Streptavidin to each well.Incubate 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Andere Bezeichnung: PYK2 (PTK2B Produkte)

Synonyme: CADTK ELISA Kit, CAKB ELISA Kit, FADK2 ELISA Kit, FAK2 ELISA Kit, PKB ELISA Kit, PTK ELISA Kit, PYK2 ELISA Kit, RAFTK ELISA Kit, CAKbeta ELISA Kit, E430023O05Rik ELISA Kit, Raftk ELISA Kit, Pyk2 ELISA Kit, fak1b ELISA Kit, ptk2.2 ELISA Kit, ptk2b ELISA Kit, si:ch1073-355e8.1 ELISA Kit, protein tyrosine kinase 2 beta ELISA Kit, PTK2 protein tyrosine kinase 2 beta ELISA Kit, protein tyrosine kinase 2aa ELISA Kit, PTK2B ELISA Kit, Ptk2b ELISA Kit, ptk2aa ELISA Kit

Hintergrund: Protein tyrosine kinase 2 beta, PTK2B, CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK

Gen-ID: 2185

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling