PKC delta ELISA Kit

Produktnummer ABIN8085853

Maus PKC delta ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus PKC delta.

Kurzübersicht für PKC delta ELISA Kit (ABIN8085853)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails PKC delta ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Mouse Protein Kinase C Delta (PKCd) ELISA Kit for detecting PKCd in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.063 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Andere Bezeichnung: Protein Kinase C Delta

Hintergrund: Gene Aliases: PKC-D, MAY1, PRKCD, SDK1, NPKC-Delta, Tyrosine-protein kinase PRKCD, Sphingosine-dependent protein kinase-1

Gen-ID: 18753

UniProt: P28867

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism