PKC alpha ELISA Kit
Kurzübersicht für PKC alpha ELISA Kit (ABIN8085847)
Target
Alle PKC alpha (PKCa) ELISA Kits anzeigenBindungsspezifität
Reaktivität
Nachweismethode
Methodentyp
Applikation
Proben
-
-
Verwendungszweck
- Phospho-PKC α (Thr638) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PKC α (Thr638) in researchers plate their cell line of choice.
-
Analytische Methode
- Qualitative
-
Bestandteile
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Benötigtes Material
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
Applikationshinweise
- Optimal working dilution should be determined by the investigator.
-
Kommentare
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Testdauer
- 4 - 5 h
-
Plattentyp
- Pre-coated
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- 4 °C
-
Informationen zur Lagerung
- 4°C for one month, Long term: see manual.
-
Haltbarkeit
- 6 months
-
-
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
-
Andere Bezeichnung
- PKC alpha
-
Hintergrund
- Gene Aliases: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C alpha type
-
Gen-ID
- 5578
-
UniProt
- P17252
-
Pathways
- WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target Alle PKC alpha (PKCa) ELISA Kits anzeigen
-
