NOD2 CLIA Kit

Produktnummer ABIN8084614

Human NOD2 ELISA Kit, Chemiluminescent Assay für die Quantifizierung von Human NOD2.

Kurzübersicht für NOD2 CLIA Kit (ABIN8084614)

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 41.2 pg/mL - 30000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails NOD2 CLIA Kit

Untere Nachweisgrenze: 41.2 pg/mL

Verwendungszweck: Human Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) CLIA Kit for detecting NOD2 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 17.4 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Andere Bezeichnung: Nucleotide Binding Oligomerization Domain Containing Protein 2

Hintergrund: Gene Aliases: CD, ACUG, BLAU, CARD15, CLR16.3, IBD1, NLRC2, NOD2B, PSORAS1, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1

Gen-ID: 64127

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome