NLRC4 ELISA Kit

Produktnummer ABIN8084447

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human NLRC4.

Kurzübersicht für NLRC4 ELISA Kit (ABIN8084447)

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails NLRC4 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human NLR Family, CARD Domain Containing Protein 4 (NLRC4) ELISA Kit for detecting NLRC4 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.056 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Andere Bezeichnung: NLR Family, CARD Domain Containing Protein 4

Hintergrund: Gene Aliases: CARD12, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, IPAF, Ice protease-activating factor, Caspase Recruitment Domain Family Member 12, NOD-Like Receptor C4

Gen-ID: 58484

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome