Leptin CLIA Kit

Produktnummer ABIN8082791

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Maus Leptin.

Kurzübersicht für Leptin CLIA Kit (ABIN8082791)

Target: Leptin (LEP)

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 41.2 pg/mL - 30000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Leptin CLIA Kit

Untere Nachweisgrenze: 41.2 pg/mL

Verwendungszweck: Mouse Leptin (LEP) CLIA Kit for detecting LEP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 13.9 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Gene Aliases: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein

Gen-ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process