Leptin ELISA Kit
Kurzübersicht für Leptin ELISA Kit (ABIN8082783)
Target
Alle Leptin (LEP) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.625 ng/mL
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Verwendungszweck
- Horse Leptin (LEP) ELISA Kit for detecting LEP in serum, plasma
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Analytische Methode
- Quantitative
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Sensitivität
- 0.243 ng/mL
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Bestandteile
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Benötigtes Material
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Leptin (LEP) ELISA Kit
LEP Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reaktivität: Kaninchen Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Maus Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Kaninchen Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Huhn Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reaktivität: Rind (Kuh) Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Probenmenge
- 100 μL
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Testdauer
- 3 h
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Plattentyp
- Pre-coated
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Testpräzision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C for one month, Long term: see manual.
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Haltbarkeit
- 12 months
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- Leptin (LEP)
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Andere Bezeichnung
- Leptin
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Hintergrund
- Gene Aliases: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
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UniProt
- Q9TU09
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Pathways
- JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target Alle Leptin (LEP) ELISA Kits anzeigen
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