SMAD4 ELISA Kit

Produktnummer ABIN8070820

Das Human SMAD4 ELISA Kit (ABIN8070820) ist ein Colorimetric ELISA Kit zur Detektion von Human SMAD4.

Kurzübersicht für SMAD4 ELISA Kit (ABIN8070820)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Bindungsspezifität: SUMOylated, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails SMAD4 ELISA Kit

Verwendungszweck: Measures sumoylated SMAD4 and total SMAD4 in human cell and tissue lysates, enabling precise assessment of SMAD4 sumoylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytische Methode: Quantitative

Bestandteile:

Size / Qty
• Microplate Coated With Anti-Pan-SMAD4 Antibody
• Anti-SUMOylation Detection Antibody
• Anti-SMAD4 Detection Antibody
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed.
2. Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund:

Gene Symbols: SMAD4, DPC4, MADH4

Background: The SUMOylated-SMAD4 and Total-SMAD4 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human sumoylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan SMAD4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SMAD4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-sumoylation antibody is used to detect sumoylated SMAD4 or biotinylated anti-SMAD4 antibody is used to detect total SMAD4 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of sumoylated SMAD4 or total SMAD4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Mothers against decapentaplegic homolog 4 (MAD homolog 4, Mothers against DPP homolog 4), Deletion target in pancreatic carcinoma 4, SMAD family member 4 (SMAD 4, Smad4, hSMAD4)

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie