PGLYRP1 ELISA Kit

Produktnummer ABIN8055871

Das Human PGLYRP1 ELISA Kit (ABIN8055871) ist ein Colorimetric ELISA Kit zur Detektion von Human PGLYRP1.

Kurzübersicht für PGLYRP1 ELISA Kit (ABIN8055871)

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Bindungsspezifität: AA 22-196

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails PGLYRP1 ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Verwendungszweck: Sandwich Human PGLYRP1 ELISA Kit to quantitate Human PGLYRP1 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: Q22-P196

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 150 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 2.9%, 4.8%, 3.7%
Inter-Assay Precision (CV%): 7.4%, 3.1%, 5.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu PGLYRP1

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Andere Bezeichnung: PGLYRP

Hintergrund:

Background: Peptidoglycan recognition protein 1, PGLYRP1, also known as TAG7, is an antibacterial and pro-inflammatory innate immunity protein that in humans is encoded by the PGLYRP1 gene. Peptidoglycan recognition proteins (PGRPs) are molecules that recognize peptidoglycan, a large component in bacterial cell walls. In insects, PGRPs activate antimicrobial pathways, and in mammals PGRPs function as antibacterial neutrophil proteins. PGRP-L halts bacterial growth by acting as an alanine amidase, an enzyme that hydrolyzes the amide bond of bacterial peptidoglycan. PGRP-I and PGRP-I are also members of the PGRP family that help recognize bacteria by binding to peptidoglycan and Gram-positive bacteria, but they do not have amidase activity. PGRP-S participates in intracellular killing of Gram-positive bacteria by stimulating two antimicrobial defense systems, the prophenoloxidase cascade and the antimicrobial peptides through Toll receptors.

Gene Full Name: peptidoglycan recognition protein 1

Gen-ID: 8993

UniProt: O75594

Pathways: Activation of Innate immune Response