PGLYRP1 ELISA Kit

Produktnummer ABIN5510755

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus PGLYRP1.

Kurzübersicht für PGLYRP1 ELISA Kit (ABIN5510755)

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Bindungsspezifität: AA 19-182

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails PGLYRP1 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Mouse PGLYRP1/Pgrps ELISA Kit to quantitate Mouse Pglyrp1 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: F19-E182

Capture antibody and Detection antibody: a monoclonal antibody from rat, a biotinylated polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5.5%, 7.2%, 5.5%
Inter-Assay Precision (CV%): 5.5%, 7.7%, 5.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu PGLYRP1

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Andere Bezeichnung: Pglyrp1

Hintergrund:

Background: Peptidoglycan recognition protein 1, also known as TAG7, is a protein that in humans is encoded by the PGLYRP1 gene. The PGLYRP1 gene is highly expressed in bone marrow, circulating Polymorphonuclear leukocytes (PMLs), and in the corneal epithelium. The PGLYRP1 protein is primarily found in the granules of PMLs. The PGLYRP1 protein plays an important role in the innate immune response. It is bactericidal against gram-positive bacteria such as S. aureus, S. epidermidis, and L. monocytogenes and generally has proinflammatory effects, inducing TNF-a and IFN-g in many tissues. PGLYRP-1 is also known to form a cytotoxic complex with HSP-70, suggesting it may also have a role in anti-cancer defense. As a pathogen recognition protein with antimicrobial properties, PGLYRP-1 is suspected to play an important role in maintaining the gut microbial flora.

Gene Full Name: peptidoglycan recognition protein 1

Gen-ID: 21946

UniProt: O88593

Pathways: Activation of Innate immune Response