Leptin ELISA Kit
Kurzübersicht für Leptin ELISA Kit (ABIN7833000)
Target
Alle Leptin (LEP) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
-
-
Untere Nachweisgrenze
- 312 pg/mL
-
Verwendungszweck
- Sandwich ELISA for quantitative detection of Mouse Leptin concentrations.
-
Analytische Methode
- Quantitative
-
Sensitivität
- 103 pg/mL
-
Bestandteile
-
- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
-
Benötigtes Material
-
- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
-
-
Leptin (LEP) ELISA Kit
LEP Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Kaninchen Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Maus Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Kaninchen Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reaktivität: Huhn Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reaktivität: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reaktivität: Schwein Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
-
Probenmenge
- 100 μL
-
Plattentyp
- Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Handhabung
- Avoid freeze/thaw cycles
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
-
Haltbarkeit
- 12 months
-
-
- Leptin (LEP)
-
Andere Bezeichnung
- LEP
-
Hintergrund
- Alternative Names: OB, OBS, Leptin, Obese protein, Obesity factor
-
UniProt
- P41160
-
Pathways
- JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target Alle Leptin (LEP) ELISA Kits anzeigen
-
