PIAS4 ELISA Kit

Produktnummer ABIN7830967

Human PIAS4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PIAS4.

Kurzübersicht für PIAS4 ELISA Kit (ABIN7830967)

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails PIAS4 ELISA Kit

Verwendungszweck: PIAS4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PIAS4 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PIAS4

Target: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Andere Bezeichnung: PIAS4

Hintergrund:

Synonyms: FLJ12419|MGC35296|PIASY|Piasg|ZMIZ6

Gene Description: protein inhibitor of activated STAT, 4

Gen-ID: 51588

Pathways: JAK-STAT Signalweg, Interferon-gamma Pathway, Positive Regulation of Response to DNA Damage Stimulus