TRADD ELISA Kit

Produktnummer ABIN7830950

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human TRADD.

Kurzübersicht für TRADD ELISA Kit (ABIN7830950)

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails TRADD ELISA Kit

Verwendungszweck: TRADD (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TRADD expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu TRADD

Target: TRADD (TNFRSF1A-Associated Via Death Domain (TRADD))

Andere Bezeichnung: TRADD

Hintergrund:

Synonyms: Hs.89862|MGC11078

Gene Description: TNFRSF1A-associated via death domain

Gen-ID: 8717

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Hepatitis C