WEE1 ELISA Kit

Produktnummer ABIN7830941

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human WEE1.

Kurzübersicht für WEE1 ELISA Kit (ABIN7830941)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails WEE1 ELISA Kit

Verwendungszweck: WEE1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of WEE1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Andere Bezeichnung: WEE1

Hintergrund:

Synonyms: DKFZp686I18166|FLJ16446|WEE1A|WEE1hu

Gene Description: WEE1 homolog (S. pombe)

Gen-ID: 7465

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase