Topoisomerase II alpha ELISA Kit

Produktnummer ABIN7830920

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Topoisomerase II alpha.

Kurzübersicht für Topoisomerase II alpha ELISA Kit (ABIN7830920)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Topoisomerase II alpha ELISA Kit

Verwendungszweck: TOP2A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TOP2A expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund:

Synonyms: TOP2|TP2A

Gene Description: topoisomerase (DNA) II alpha 170 kDa

Gen-ID: 7153

Pathways: Zellzyklus, Mitotic G1-G1/S Phases