PKC delta ELISA Kit

Produktnummer ABIN7830855

Das Human PKC delta ELISA Kit (ABIN7830855) ist ein Colorimetric ELISA Kit zur Detektion von Human PKC delta.

Kurzübersicht für PKC delta ELISA Kit (ABIN7830855)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PKC delta ELISA Kit

Verwendungszweck: PRKCD (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCD expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Andere Bezeichnung: PRKCD

Hintergrund:

Synonyms: MAY1|MGC49908|PKCD|nPKC-delta

Gene Description: protein kinase C, delta

Gen-ID: 5580

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism