PKC alpha ELISA Kit
Kurzübersicht für PKC alpha ELISA Kit (ABIN7830853)
Target
Alle PKC alpha (PKCa) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- PRKCA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCA expression in cultured cells.
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Analytische Methode
- Qualitative
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Kreuzreaktivität
- Maus, Ratte
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Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa Reaktivität: Maus Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reaktivität: Maus Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Uncoated
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Protokoll
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the kit at 4°C.
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Andere Bezeichnung
- PRKCA
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Hintergrund
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Synonyms: AAG6|MGC129900|MGC129901|PKC-alpha|PKCA|PRKACA
Gene Description: protein kinase C, alpha
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Gen-ID
- 5578
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Pathways
- WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target Alle PKC alpha (PKCa) ELISA Kits anzeigen
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