c-MYC ELISA Kit

Produktnummer ABIN7830814

Human c-MYC ELISA Kit, Colorimetric Assay für die Quantifizierung von Human c-MYC.

Kurzübersicht für c-MYC ELISA Kit (ABIN7830814)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails c-MYC ELISA Kit

Verwendungszweck: MYC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MYC expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Andere Bezeichnung: MYC

Hintergrund:

Synonyms: bHLHe39|c-Myc

Gene Description: v-myc myelocytomatosis viral oncogene homolog (avian)

Gen-ID: 4609

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt