VEGFR2/CD309 ELISA Kit

Produktnummer ABIN7830807

Das Human VEGFR2/CD309 ELISA Kit (ABIN7830807) ist ein Colorimetric ELISA Kit zur Detektion von Human VEGFR2/CD309.

Kurzübersicht für VEGFR2/CD309 ELISA Kit (ABIN7830807)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails VEGFR2/CD309 ELISA Kit

Verwendungszweck: KDR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KDR expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Andere Bezeichnung: KDR

Hintergrund:

Synonyms: CD309|FLK1|VEGFR|VEGFR2

Gene Description: kinase insert domain receptor (a type III receptor tyrosine kinase)

Gen-ID: 3791

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling