PTK2B ELISA Kit

Produktnummer ABIN7830799

Human PTK2B ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PTK2B.

Kurzübersicht für PTK2B ELISA Kit (ABIN7830799)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PTK2B ELISA Kit

Verwendungszweck: PTK2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTK2B expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Andere Bezeichnung: PTK2B

Hintergrund:

Synonyms: CADTK|CAKB|FADK2|FAK2|FRNK|PKB|PTK|PYK2|RAFTK

Gene Description: PTK2B protein tyrosine kinase 2 beta

Gen-ID: 2185

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling