IL4 Receptor ELISA Kit

Produktnummer ABIN7830606

Das Human IL4 Receptor ELISA Kit (ABIN7830606) ist ein Colorimetric ELISA Kit zur Detektion von Human IL4 Receptor.

Kurzübersicht für IL4 Receptor ELISA Kit (ABIN7830606)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails IL4 Receptor ELISA Kit

Verwendungszweck: IL4R (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL4R expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: IL4R

Hintergrund:

Synonyms: CD124|IL4RA

Gene Description: interleukin 4 receptor

Gen-ID: 3566

UniProt: P24394

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process