IL4 Receptor ELISA Kit

Produktnummer ABIN6730183

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus IL4 Receptor.

Kurzübersicht für IL4 Receptor ELISA Kit (ABIN6730183)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.33 ng/mL - 750 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails IL4 Receptor ELISA Kit

Untere Nachweisgrenze: 1.33 ng/mL

Verwendungszweck: Mouse IL-4 R alpha ELISA Kit for Plasma, Serum, and Cell Culture Supernatants samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects mouse IL-4 R alpha. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: IL-4 R alpha

Hintergrund: Gene Symbols: Interleukin-4 receptor subunit alpha (IL-4 receptor subunit alpha) (IL-4R subunit alpha) (IL-4R-alpha) (IL-4RA) (CD antigen CD124) [Cleaved into: Soluble interleukin-4 receptor subunit alpha (Soluble IL-4 receptor subunit alpha) (Soluble IL-4R-alpha) (sIL4Ralpha/prot) (IL-4-binding protein) (IL4-BP)

UniProt: P16382

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process