IGF1R ELISA Kit

Produktnummer ABIN7830595

Human IGF1R ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IGF1R.

Kurzübersicht für IGF1R ELISA Kit (ABIN7830595)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails IGF1R ELISA Kit

Verwendungszweck: IGF1R (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IGF1R expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: IGF1R

Hintergrund:

Synonyms: CD221|IGFIR|JTK13|MGC142170|MGC142172|MGC18216

Gene Description: insulin-like growth factor 1 receptor

Gen-ID: 3480

UniProt: P08069

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie