CREB1 ELISA Kit
Kurzübersicht für CREB1 ELISA Kit (ABIN7830525)
Target
Alle CREB1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- CREB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CREB1 expression in cultured cells.
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Analytische Methode
- Qualitative
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Kreuzreaktivität
- Maus, Ratte
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cAMP Responsive Element Binding Protein 1 (CREB1) ELISA Kit
CREB1 Reaktivität: Human pSer133 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Maus Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 pmol/mL - 20 pmol/mL Cell Culture Supernatant, Plasma, Serum
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Human phosphorylated Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 pmol/mL - 20 pmol/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Uncoated
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Protokoll
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the kit at 4°C.
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- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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Andere Bezeichnung
- CREB1
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Hintergrund
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Synonyms: CREB|MGC9284
Gene Description: cAMP responsive element binding protein 1
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Gen-ID
- 1385
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UniProt
- P16220
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Pathways
- TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
Target Alle CREB1 ELISA Kits anzeigen
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