BTK ELISA Kit

Produktnummer ABIN7830451

Human BTK ELISA Kit, Colorimetric Assay für die Quantifizierung von Human BTK.

Kurzübersicht für BTK ELISA Kit (ABIN7830451)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails BTK ELISA Kit

Verwendungszweck: BTK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BTK expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Andere Bezeichnung: BTK

Hintergrund:

Synonyms: AGMX1|AT|ATK|BPK|IMD1|MGC126261|MGC126262|PSCTK1|XLA

Gene Description: Bruton agammaglobulinemia tyrosine kinase

Gen-ID: 695

UniProt: Q06187

Pathways: Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling