ARAF ELISA Kit

Produktnummer ABIN7830421

Human ARAF ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ARAF.

Kurzübersicht für ARAF ELISA Kit (ABIN7830421)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ARAF ELISA Kit

Verwendungszweck: ARAF (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ARAF expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Andere Bezeichnung: ARAF

Hintergrund:

Synonyms: A-RAF|ARAF1|PKS2|RAFA1

Gene Description: v-raf murine sarcoma 3611 viral oncogene homolog

Gen-ID: 369

UniProt: P10398

Pathways: MAPK Signalweg, RTK Signalweg, Hepatitis C