CD40 Ligand ELISA Kit

Produktnummer ABIN7826579

Das Human CD40 Ligand ELISA Kit (ABIN7826579) ist ein Colorimetric ELISA Kit zur Detektion von Human CD40 Ligand.

Kurzübersicht für CD40 Ligand ELISA Kit (ABIN7826579)

Target: CD40 Ligand (CD40LG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails CD40 Ligand ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: This ELISA kit applies to the in vitro quantitative determination of Human sCD40L concentrations in serum, plasma.

Analytische Methode: Quantitative

Kreuzreaktivität (Details): This kit recognizes Human sCD40L in samples.No significant cross-reactivity or interference between Human sCD40L and analogues was observed

Produktmerkmale: Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Bestandteile:

• Capture Antibody
• Reference Standard
• Micro ELISA pre-Plate
• Biotinylated Detection Antibody
• HRP Conjugate
• Manual

Benötigtes Material:

• Ancillary Reagents (ABIN7670776)
• Microplate reader with 450 nm wavelength filter
• High-precision transfer pipette
• EP tubes
• disposable pipette tips
• Incubator capable of maintaining 37 °C
• Deionized or distilled water
• Absorbent paper
• Loading slot

Anwendungsinformationen

Probenmenge: 100 µL

Testdauer: 1 d

Plattentyp: Uncoated

Protokoll:

1. Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
   Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C
2. Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
3. Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
4. Aspirate and wash the plate 3 times
5. Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
7. Add 50 µL Stop Solution
8. Read the plate at 450 nm immediately. Calculation of the results

Testpräzision: Both intra-CV and inter-CV are < 10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Store all unopend components at -20°C for up to 12 months.

Haltbarkeit: 12 months

Details zu CD40 Ligand

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: sCD40L

Hintergrund: TNFSF5,CD40LG,CD154,HIGM1,IGM,IMD3,T-BAM,TRAP,Gp39,

UniProt: P29965

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints