Survivin ELISA Kit

Produktnummer ABIN6951658

Produktdetails Survivin ELISA Kit

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Survivin/BIRC5 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Andere Bezeichnung: Survivin (BIRC5 (BIRC5 Produkte)

Synonyme: AP14 ELISA Kit, API4 ELISA Kit, EPR-1 ELISA Kit, AAC-11 ELISA Kit, Api4 ELISA Kit, TIAP ELISA Kit, survivin40 ELISA Kit, bir5a ELISA Kit, survivin ELISA Kit, BIR1 ELISA Kit, Su2 ELISA Kit, XBIR1 ELISA Kit, XSurvivin1 ELISA Kit, Xsvv1 ELISA Kit, api4 ELISA Kit, bir1-A ELISA Kit, epr-1 ELISA Kit, svv1 ELISA Kit, xSurvivin ELISA Kit, xsurv ELISA Kit, Su1 ELISA Kit, birc5.2 ELISA Kit, svv2 ELISA Kit, xSurvivin2 ELISA Kit, bir5b ELISA Kit, SIX ELISA Kit, birc5.2-a ELISA Kit, birc5.2-b ELISA Kit, baculoviral IAP repeat containing 5 ELISA Kit, baculoviral IAP repeat-containing 5 ELISA Kit, baculoviral IAP repeat containing 5a ELISA Kit, baculoviral IAP repeat-containing 5, gene 1 L homeolog ELISA Kit, baculoviral IAP repeat containing 5 L homeolog ELISA Kit, baculoviral IAP repeat containing 5b ELISA Kit, baculoviral IAP repeat containing 5 S homeolog ELISA Kit, BIRC5 ELISA Kit, Birc5 ELISA Kit, birc5a ELISA Kit, birc5.1.L ELISA Kit, birc5.2.L ELISA Kit, birc5b ELISA Kit, birc5.2.S ELISA Kit

Gen-ID: 332

UniProt: O15392

Pathways: Apoptose, Zellzyklus, Nuclear Hormone Receptor Binding