WISP1 ELISA Kit
WISP1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951247
-
-
Target
Alle WISP1 ELISA Kits anzeigen
-
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
Reaktivität
-
Maus
-
Nachweismethode
-
Colorimetric
-
Methodentyp
-
Sandwich ELISA
-
Applikation
-
ELISA
-
Verwendungszweck
-
Mouse WISP-1 ELISA Kit.
-
Proben
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytische Methode
-
Quantitative
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product WISP1 ELISA Kit
-
-
-
Applikationshinweise
-
Optimal working dilution should be determined by the investigator.
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Haltbarkeit
-
6 months
-
-
Target
Alle WISP1 ELISA Kits anzeigen
-
WISP1
(WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
-
Andere Bezeichnung
-
WISP-1 (WISP1 Produkte)
-
Synonyme
-
CCN4 ELISA Kit, WISP1c ELISA Kit, WISP1i ELISA Kit, WISP1tc ELISA Kit, WISP-1 ELISA Kit, WISP1 ELISA Kit, si:ch211-38f10.2 ELISA Kit, AW146261 ELISA Kit, Elm1 ELISA Kit, zgc:172276 ELISA Kit, WNT1 inducible signaling pathway protein 1 ELISA Kit, WNT1 inducible signaling pathway protein 1a ELISA Kit, WNT1-inducible-signaling pathway protein 1-like ELISA Kit, WNT1 inducible signaling pathway protein 1b ELISA Kit, WISP1 ELISA Kit, wisp1a ELISA Kit, Wisp1 ELISA Kit, LOC100627044 ELISA Kit, wisp1b ELISA Kit
-
Gen-ID
-
22402
-
UniProt
-
O54775
-
Pathways
-
WNT Signalweg, Growth Factor Binding
-