IL-18 ELISA Kit
Kurzübersicht für IL-18 ELISA Kit (ABIN6951157)
Target
Alle IL-18 (IL18) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
-
-
Verwendungszweck
- Feline IL-18 ELISA Kit.
-
Analytische Methode
- Quantitative
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
-
Applikationshinweise
- Optimal working dilution should be determined by the investigator.
-
Plattentyp
- Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Haltbarkeit
- 6 months
-
-
- IL-18 (IL18) (Interleukin 18 (IL18))
-
Andere Bezeichnung
- IL-18
-
UniProt
- M3WT43
-
Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target Alle IL-18 (IL18) ELISA Kits anzeigen
-