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HSPD1 ELISA Kit

HSPD1 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951140
$734.80
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 21 bis 31 Werktagen
  • Target Alle HSPD1 ELISA Kits anzeigen
    HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
    Reaktivität
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse HSPD1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle HSPD1 ELISA Kits anzeigen
    HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
    Andere Bezeichnung
    HSPD1 (HSPD1 Produkte)
    Synonyme
    CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, chaperonin, cpn60, groel, hld4, hsp60, hsp65, spg13, 60kDa, Hsp60, 12, BP5, CG12101, Cpn60, Dmel\\CG12101, Dmhsp60, G62, HSP60A, Hsp60A, IEF16, Mmp-P1, SSP 7506, d-hsp60, hsp60A, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, cb863, fa04a05, fb22d10, fi27b05, id:ibd2197, sb:cb144, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05, MIF4, MNA2, mopA, groL, crpA, Hspd1-30p, heat shock protein family D (Hsp60) member 1, heat shock protein family D (Hsp60) member 1 S homeolog, 60 kDa heat shock protein, mitochondrial, heat shock protein 1 (chaperonin), Heat shock protein 60A, heat shock 60 protein 1, chaperone ATPase HSP60, molecular chaperone GroEL, thermosome subunit, chaperonin GroEL, mitochondrial chaperonin, heat shock protein family D member 1, HSPD1, hspd1.S, hspd1, LOC100414401, Hspd1, Hsp60A, HSP60, groEL, MMP_RS07785, groEl, LOC100136430
    Gen-ID
    15510
    UniProt
    P63038
    Pathways
    Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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