Complement Factor H ELISA Kit

Produktnummer ABIN6730142

Produktdetails Complement Factor H ELISA Kit

Target: Complement Factor H (CFH)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Complement Factor H ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse Complement Factor H.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: Complement Factor H (CFH Produkte)

Synonyme: AHUS1 ELISA Kit, AMBP1 ELISA Kit, ARMD4 ELISA Kit, ARMS1 ELISA Kit, CFHL3 ELISA Kit, FH ELISA Kit, FHL1 ELISA Kit, HF ELISA Kit, HF1 ELISA Kit, HF2 ELISA Kit, HUS ELISA Kit, Mud-1 ELISA Kit, NOM ELISA Kit, Sas-1 ELISA Kit, Sas1 ELISA Kit, Fh ELISA Kit, CFH ELISA Kit, im:7147412 ELISA Kit, si:ch211-207o17.2 ELISA Kit, fH ELISA Kit, armd4 ELISA Kit, arms1 ELISA Kit, cfh ELISA Kit, cfhl3 ELISA Kit, fhl1 ELISA Kit, hf1 ELISA Kit, hf2 ELISA Kit, hus ELISA Kit, complement factor H ELISA Kit, complement component factor h ELISA Kit, complement factor H-related protein 5 ELISA Kit, complement factor H L homeolog ELISA Kit, CFH ELISA Kit, Cfh ELISA Kit, cfh ELISA Kit, LOC100399888 ELISA Kit, LOC100733902 ELISA Kit, cfh.L ELISA Kit

UniProt: P06909

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin