Complement Factor H ELISA Kit

Produktnummer ABIN6720016

Produktdetails Complement Factor H ELISA Kit

Target: Complement Factor H (CFH)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H isoform b, Factor H, factor H like 1, FH, FHL 1, FHL1, H factor 1 (complement)|H factor 1, H factor 2 (complement), HF 1, HF 2, HF, HF1, HF2, HUS, MGC88246

Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Gene Name: CFH

Production: Natural and recombinant mouse CFH. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S875-V1252

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CFH standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: Complement H - CFH (CFH Produkte)

Synonyme: AHUS1 ELISA Kit, AMBP1 ELISA Kit, ARMD4 ELISA Kit, ARMS1 ELISA Kit, CFHL3 ELISA Kit, FH ELISA Kit, FHL1 ELISA Kit, HF ELISA Kit, HF1 ELISA Kit, HF2 ELISA Kit, HUS ELISA Kit, Mud-1 ELISA Kit, NOM ELISA Kit, Sas-1 ELISA Kit, Sas1 ELISA Kit, Fh ELISA Kit, CFH ELISA Kit, im:7147412 ELISA Kit, si:ch211-207o17.2 ELISA Kit, fH ELISA Kit, armd4 ELISA Kit, arms1 ELISA Kit, cfh ELISA Kit, cfhl3 ELISA Kit, fhl1 ELISA Kit, hf1 ELISA Kit, hf2 ELISA Kit, hus ELISA Kit, complement factor H ELISA Kit, complement component factor h ELISA Kit, complement factor H-related protein 5 ELISA Kit, complement factor H L homeolog ELISA Kit, CFH ELISA Kit, Cfh ELISA Kit, cfh ELISA Kit, LOC100399888 ELISA Kit, LOC100733902 ELISA Kit, cfh.L ELISA Kit

Gen-ID: 12628

NCBI Accession: NP_034018

UniProt: P06909

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin