Amphiregulin ELISA Kit

Produktnummer ABIN6720359

Hersteller Produkt- Nr.: koa0144

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Amphiregulin.

Kurzübersicht für Amphiregulin ELISA Kit (ABIN6720359)

Target: Amphiregulin (AREG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Produktdetails Amphiregulin ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Hersteller Produkt- Nr.: koa0144

Hersteller: Rockland

Verwendungszweck: Human Amphiregulin (AR) Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Amphiregulin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Amphiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. It is recommended that each human Amphiregulin standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: S101-K198

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Amphiregulin

Target: Amphiregulin (AREG)

Andere Bezeichnung: Amphiregulin(AR)

Hintergrund:

Synonyms: A REG, AR, AREG, Colorectum cell derived growth

Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. Amphiregulin is related to epidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

Gen-ID: 374

NCBI Accession: NP_001648

UniProt: P15514

Pathways: RTK Signalweg, EGFR Signaling Pathway