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Amphiregulin ELISA Kit

AREG Reaktivität: Human AA 101-198 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Produktnummer ABIN1889290
  • Target Alle Amphiregulin (AREG) ELISA Kits anzeigen
    Amphiregulin (AREG)
    Bindungsspezifität
    AA 101-198
    Reaktivität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6-1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Amphiregulin(AR)
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: S101-K198
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: S101-K198
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the amphiregulin family.

    Plattentyp
    Pre-coated
    Protokoll
    human Amphiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been precoated onto 96-well plates. Standards(E.coli, S101-K198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Amphiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Amphiregulin amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Amphiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Amphiregulin standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 6.67, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 351, Standard deviation: 15.1, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 633, Standard deviation: 29.75, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 104, Standard deviation: 8.94, CV(%): 8.6
    • Sample 2: n=24, Mean(pg/ml): 339, Standard deviation: 24.75, CV(%): 7.3
    • Sample 3: n=24, Mean(pg/ml): 620, Standard deviation: 32.24, CV(%): 5.2
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle Amphiregulin (AREG) ELISA Kits anzeigen
    Amphiregulin (AREG)
    Andere Bezeichnung
    AREG (AREG Produkte)
    Synonyme
    AR ELISA Kit, CRDGF ELISA Kit, SDGF ELISA Kit, Sdgf ELISA Kit, AREG ELISA Kit, AREGB ELISA Kit, AREG/SDGF ELISA Kit, amphiregulin ELISA Kit, AREG ELISA Kit, Areg ELISA Kit
    Hintergrund

    Protein Function: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

    Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

    Synonyms: Amphiregulin,AR,Colorectum cell-derived growth factor,CRDGF,AREG,SDGF,AREGB,

    Full Gene Name: Amphiregulin

    Cellular Localisation: Membrane, Single-pass membrane protein.
    Gen-ID
    374
    UniProt
    P15514
    Pathways
    RTK Signalweg, EGFR Signaling Pathway
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