Kallikrein 5 ELISA Kit

Produktnummer ABIN6720358

Hersteller Produkt- Nr.: koa0487

Human Kallikrein 5 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Kallikrein 5.

Kurzübersicht für Kallikrein 5 ELISA Kit (ABIN6720358)

Target: Kallikrein 5 (KLK5)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum

Produktdetails Kallikrein 5 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Hersteller Produkt- Nr.: koa0487

Hersteller: Rockland

Verwendungszweck: Human KLK5 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: V23-S293

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human KLK5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), human milk or saliva to each empty well. We recommend that each human KLK5 standard solution and each sample is measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 4%, 4.2%, 6.2%
Inter-Assay Precision (CV%): 5.7%, 4.3%, 6.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Kallikrein 5

Target: Kallikrein 5 (KLK5)

Andere Bezeichnung: KLK5

Hintergrund:

Background: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Gene Full Name: kallikrein related peptidase 5

Gen-ID: 25818

NCBI Accession: NP_001070959

UniProt: Q9Y337

Pathways: Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling