Kallikrein 5 ELISA Kit

Produktnummer ABIN1672807

Produktdetails Kallikrein 5 ELISA Kit

Target: Kallikrein 5 (KLK5)

Bindungsspezifität: AA 23-293

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK5

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk, Saliva

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: V23-S293

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V23-S293

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Tissue Specificity: Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .

Plattentyp: Pre-coated

Protokoll: human KLK5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK5 has been precoated onto 96-well plates. Standards(NSO, V23-S293) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK5 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human KLK5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), human milk or saliva to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human KLK5 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 38.7, CV(%): 7.1
• Sample 2: n=16, Mean(pg/ml): 1823, Standard deviation: 69.3, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 3240, Standard deviation: 158.8, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 613, Standard deviation: 46.6, CV(%): 7.6
• Sample 2: n=24, Mean(pg/ml): 1972, Standard deviation: 98.6, CV(%): 5
• Sample 3: n=24, Mean(pg/ml): 3468, Standard deviation: 260.1, CV(%): 7.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Kallikrein 5

Target: Kallikrein 5 (KLK5)

Andere Bezeichnung: KLK5 (KLK5 Produkte)

Synonyme: KLK-L2 ELISA Kit, KLKL2 ELISA Kit, SCTE ELISA Kit, 1110030O19Rik ELISA Kit, KAL ELISA Kit, KALA ELISA Kit, Klk1 ELISA Kit, Klk5 ELISA Kit, RATKALA ELISA Kit, kallikrein related peptidase 5 ELISA Kit, kallikrein related-peptidase 5 ELISA Kit, kallikrein 5-like ELISA Kit, KLK5 ELISA Kit, Klk5 ELISA Kit, Klk5l ELISA Kit

Hintergrund:

Protein Function: May be involved in desquamation.

Background: Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Synonyms: Kallikrein-5,3.4.21.-,Kallikrein-like protein 2,KLK-L2,Stratum corneum tryptic enzyme,KLK5,SCTE,UNQ570/PRO1132,

Full Gene Name: Kallikrein-5

Cellular Localisation: Secreted.

Gen-ID: 25818

UniProt: Q9Y337

Pathways: Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling