IL15RA ELISA Kit

Produktnummer ABIN6720034

Hersteller Produkt- Nr.: koa0654

Maus IL15RA ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL15RA.

Kurzübersicht für IL15RA ELISA Kit (ABIN6720034)

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails IL15RA ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Hersteller Produkt- Nr.: koa0654

Hersteller: Rockland

Verwendungszweck: Mouse IL-15RA Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse IL15RA. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: G33-K205

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IL15RA

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Andere Bezeichnung: IL15RA

Hintergrund:

Synonyms: AA690181, CD215, I15RA_HUMAN, IL 15R alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA, Interleukin 15 receptor alpha, Interleukin 15 receptor subunit alpha, MGC104179, sIL-15 receptor subunit alpha, sIL-15R-alpha, sIL-15RA, Soluble interleukin 15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha

Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Gen-ID: 16169

NCBI Accession: NP_001258426

UniProt: Q60819

Pathways: JAK-STAT Signalweg