IL15RA ELISA Kit

Produktnummer ABIN6730186

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human und Maus IL15RA.

Kurzübersicht für IL15RA ELISA Kit (ABIN6730186)

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.045 ng/mL - 12.5 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails IL15RA ELISA Kit

Untere Nachweisgrenze: 0.045 ng/mL

Verwendungszweck: Mouse IL-15 R alpha ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse ADAM9, CD14, CD39L3, CDNF, Cripto, CXCL14, Epimorphin, Erythropoietin R, Flt-3, Follistatin, Frizzled-1, Frizzled-4, FSTL1, GDF-3, GDF-7, GFR alpha-3, IFN-gamma R2, IL-1 RI, IL-1 RII, IL-10 R beta, IL-11 R alpha, IL-17 RA, IL-17 RC, IL-20 R beta, IL-1F8, IL-21 R, IL23 R, IL-28A, JAM-C, Klotho beta, Layilin, LDL R, LIF, Matrilin-3, Lymphotoxin beta R, Nephrin, Neurocan, NKp46, Laminin alpha 4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu IL15RA

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Andere Bezeichnung: IL-15 R alpha

Hintergrund:

Gene Symbols: IL15RA

Protein Name (Synonyms): Interleukin-15 receptor subunit alpha (IL-15 receptor subunit alpha) (IL-15R-alpha) (IL-15RA) (CD antigen CD215) [Cleaved into: Soluble interleukin-15 receptor subunit alpha (sIL-15 receptor subunit alpha) (sIL-15R-alpha) (sIL-15RA)

Gen-ID: 16169

UniProt: Q60819

Pathways: JAK-STAT Signalweg