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Soluble E-Selectin ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Soluble E-Selectin in Cell Culture Supernatant, Plasma (heparin - citrate) und Serum.
Rockland
Produktnummer ABIN6719948
Hersteller Produkt- Nr.: koa0298

Kurzübersicht für Soluble E-Selectin ELISA Kit (ABIN6719948)

Target

Alle Soluble E-Selectin (sE-Selectin) Produkte
Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Reaktivität

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Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5 pg/mL - 4000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (heparin - citrate), Serum
  • Untere Nachweisgrenze

    62.5 pg/mL

    Hersteller Produkt- Nr.

    koa0298

    Hersteller

    Rockland

    Verwendungszweck

    Mouse sE-Selectin Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant mouse sE-Selectin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse E-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, citrate) to each empty well. It is recommended that each mouse E-Selectin standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: W22-P557

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle Soluble E-Selectin (sE-Selectin) Produkte

    Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

    Andere Bezeichnung

    sE-Selectin

    Hintergrund

    Synonyms: CD62 antigen-like family member E, CD62E, CD62E antigen, E selectin, E-selectin, ELAM, ELAM-1, ELAM1, Endothelial leukocyte adhesion molecule 1, ESEL, LECAM2, Leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2, LYAM2_HUMAN, sele

    Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.

    Gen-ID

    20339

    NCBI Accession

    NP_035475

    UniProt

    Q00690
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