Soluble E-Selectin ELISA Kit

Produktnummer ABIN1672750

Maus Soluble E-Selectin ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus Soluble E-Selectin und es wurde in 3+ Publikationen zitiert.

Kurzübersicht für Soluble E-Selectin ELISA Kit (ABIN1672750)

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Bindungsspezifität: AA 22-557

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Produktdetails Soluble E-Selectin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Mouse sE-Selectin ELISA Kit to quantitate Mouse Sele in cell culture supernatants, serum and plasma (heparin, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: W22-P557

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.4%, 6.7%, 4.3%
Inter-Assay Precision (CV%): 6%, 7.5%, 5.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Soluble E-Selectin ELISA Kit (ABIN1672750)

Li, Ben, Yuan, Liu, Wu, Xu, Zhang: "Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates." in: Emerging microbes & infections, Vol. 2, Issue 7, pp. e42, (2015) (PubMed).

Zhong, He, Yang, Bao, Zhang, Zhou, Chen, Li, Han, Yang, Sun, Cao, Li, Shi, Jiang, Zhang, Yan: "L-selectin and P-selectin are novel biomarkers of cervicovaginal inflammation for preclinical mucosal safety assessment of anti-HIV-1 microbicide." in: Antimicrobial agents and chemotherapy, Vol. 56, Issue 6, pp. 3121-32, (2012) (PubMed).

Iori, Vinci, Murphy, Marescotti, Avogaro, Ahluwalia: "Glucose and fatty acid metabolism in a 3 tissue in-vitro model challenged with normo- and hyperglycaemia." in: PLoS ONE, Vol. 7, Issue 4, pp. e34704, (2012) (PubMed).

Details zu Soluble E-Selectin

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Andere Bezeichnung: sE-Selectin

Hintergrund:

Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.

Gene Full Name: selectin, endothelial cell

Gen-ID: 20339

UniProt: Q00690